Figure 3.

Pulsed Field Gel Electrophoresis resolved L. amazonensis chromosomes hybridized with chromosome-specific markers. Following PFGE, gels were blotted and hybridized to radiolabeled probes (A) oligopeptidase; (B) spliced leader RNA gene, (C), DHRF-TS and (D), the genomic fragment from a cosmid clone10E07. Each lane corresponds to one L. amazonensis strain, identified by the number reported in Table 1. Lb, L. braziliensis ; F, L. major ; Molecular size is shown at the left side of each panel, (Mb). L. major chromosome numbers of interest are numbered in the figure.