Figure 3.

Specificity of the polymerase chain reaction for T. evansi. The 810-bp PCR amplification product was not detected from 1.0 ng of DNA extracted from Babesia and Theileria spp. Lane MW: molecular weight marker (1 Kb ladder); Lane 1: 1.0 ng of T. evansi DN (positive control), Lane 2: 1.0 pg DNA of Theileria anulata, Lane 3: 1.0 pg DNA of Babesia bigemina, Lane 4: nucleic acid free sample (negative control).