Figure 3.

Immunoblot of LmxMPK4 from L. mexicana wild type and episomal overexpressor. Lane 1, L. mexicana wild type promastigotes (4 × 10⁷ cells); lane 2, amastigotes derived from mouse lesion (4 × 10⁷ cells); lane 3, wild type in vitro differentiated amastigotes (1 × 10⁸ cells); lane 4, promastigotes of LmxMPK4 episomal overexpressor (1 × 10⁷ cells). A, blot probed with antiserum against the carboxy-terminal peptide of LmxMPK4; B, the blot was stripped and re-probed with a polyclonal antiserum against myo-inositol-1-phosphate synthase. The molecular masses of standard proteins are indicated in kilodaltons.